Nanjing Pars Biochem CO.,Ltd

FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.

Purpose

This kit allows for the determination of CALP concentrations in Chicken serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Chicken CALP level in the sample，use Purified Chicken CALP antibody to coat microtiter plate wells, make solid-phase antibody, then add CALP to wells, Combined CALP antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CALP   in the samples is then determined by comparing the O.D. of the samples to the standard curve